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Chuckles, Flash, Rampart, Hardball, Skidmark, Tracker (or Action Man, whichever), Lady Jaye, Mainframe
I dropped the International characters from this list, because they're almost as obscure and meaningless as comic-created characters. And for clarity:
Recondo faked his death, but contacted Sparks at the end of the issue (SM: Tokyo) to let him know he was still alive and kicking.
Tracker (Chris Groen) was killed in the run-up to the Red Shadows arc. The Action Man line was a nod to Tracker's being dropped from the Night Force 5-pack that same year, in favor of Action Man using the same mold. It was a bad in-joke that fell very, very flat.
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Big Boa, Croc Master 2, Overlord, Serpentor II, Dr. Mindbender II, Monkeywrench, Cesspool
Also Big Boa, Sky Creeper, Rip-It, Headman, and Overkill.
General Rey did get his origin explained in America's Elite #19-20. In addition to 9 Serpentots, Hannibal, and the original Serpentor, there was a second adult clone that Mindbender sold to the Jugglers years ago (probably when he blackmailed them into sending the Joes to Cobra Island in Marvel #74-77). Mike O'Sullivan also un-retconned Serpentor's death (he *was* buried in the Freighter and burned to ask when Cobra abandoned the Island). The Coil created a new clone using the original DNA samples, and led Serpentor II to believe he was the original. The second Serpentor was in the tank in the Juggler's HQ when Mars Harring blew the place to smitherinies!
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What?!? Well, that just takes all the fun out of it! Where's the risk in that?
A lot of Brandon Jerwa's ideas got chopped down when the first DDP ongoing was DX'ed. Chuckles' death, we've long known, was supposed to be part of a larger plot (the death was faked, btw) that Jerwa never got to explore. When you consider Mikhail Derenko (Overlord) was originally part of Duke and Chuckles' Hammer Team (from Master and Apprentice), and Overlord was the guy to take out Chuckles on Cobra Island, there's definitely something more going on there (having the covert ops guy on the frontlines was purposely done by Jerwa, yet most readers pan him for it's military innacuracy).
Rey's storyline *was* supposed to be a lot bigger, and I'm not sure we've ever really gotten a full explanation of the original plan, but Mike O'Sullivan had to wrap up that plotpoint and he chose the safe route.